Extraction of anthocyanins from haskap berry pulp using supercritical carbon dioxide: Influence of co-solvent composition and pretreatment
Guangling Jiao, Azadeh Kermanshahi pour∗
Biorefining and Remediation Laboratory, Department of Process Engineering and Applied Science, Dalhousie University, PO, 15000, Halifax, Nova Scotia, B3H 4R2,
Extracts rich in anthocyanin compounds were obtained from haskap berry pulp paste using supercritical carbon dioxide (scCO2) and water as co-solvent. The extraction conditions including pressure, temperature, and amount of water were further optimized by Box-Behnken design. The highest total anthocyanins (TA) yield of 52.7% was
achieved at 45 MPa, 65 °C, 5.4 g water to 3.2 g berry pulp paste, 15 min static and 20 min dynamic time. Different combinations of water and ethanol as co-solvent did not significantly affect the TA yield. Furthermore, similar anthocyanin extraction yields were obtained in the case of the pulp paste and rehydrated freeze-dried berry pulp powder, which indicates that freeze drying pretreatment is not required prior to scCO2 extraction.
Compared with conventional extraction, the use of scCO2 and water as co-solvent offered higher anthocyanin extraction efficiency (52.7% versus 38.3%) with improved antioxidant activity (89.8% versus 72.2%).