AP-224 – Supercritical CO2 extraction of valuable lipophilic compounds from pre-fractionated sour cherry pomace and evaluation of their composition and properties

AP-224 – Supercritical CO2 extraction of valuable lipophilic compounds from pre-fractionated sour cherry pomace and evaluation of their composition and properties

Laura Jūrienė, Vita Morkūnienė, Petras Rimantas Venskutonis

Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų pl. 19, Kaunas LT-50254, Lithuania
Received 26 March 2024, Revised 28 July 2024, Accepted 29 July 2024, Available online 31 July 2024, Version of Record 31 July 2024.

Highlights
• Sour cherry pomace was separated into six different particle size fractions.
• The fractions were extracted with supercritical CO2 yielding 3.38−8.69 % of lipids.
• Linoleic (38.52−47.11 %) and oleic (21.85−39.03 %) fatty acids were major in the oils.
• The concentrations of tocopherols, phytosterols and carotenoids were determined.
• ORAC values of fraction extracts of were 35.12−95.49 of mg trolox equivalents/g.

Abstract

Valorisation of fruit processing by-products and waste is an important task for increasing the sustainability of agro-food sector. In this study, pitted sour cherry pomace was mechanically pre-fractionated into the 6 different particle size (>10, 4−10, 3−4, 1−3, 0.8−1, <0.8 mm) fractions (9.71−16.41 % proteins, 5.68−12.16 % fat, 62.55−78.39 % carbohydrates) and subjected to supercritical fluid extraction with CO2 for the recovery of lipophilic constituents. Extract yield depended on fat content and was from 3.38 % to 8.69 %. Linoleic (38.52−47.11 %) and oleic (21.85−39.03 %) were major fatty acids, while triacylglycerols composed of these acids were major in the extracted oils. The concentrations of tocopherols, carotenoids and phytosterols in the extracts were 116.3−432.0, 1218−2564 and 4294−8449 μg/g. Antioxidant activity values were determined for the extracts and solids of initial dry pomace and its residue after extraction. Folin-Ciocalteu Index (basically similar to total phenolic content, TPC), ABTS•+-scavenging and oxygen radical absorbance (ORAC) values of extracts were 7.86−8.75 mg of gallic acid equivalents/g, 1.72−6.37 and 35.12−95.49 of mg trolox equivalents/g, respectively. It is the first report on comprehensive characterisation of sour cherry pomace fractions extracted by supercritical CO2.